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Conjugate
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验证数据展示

  • WB analysis of HeLa using 28983-1-AP (same clone as 28983-1-PBS)

    WB analysis of HeLa using 28983-1-AP (same clone as 28983-1-PBS)

    Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28983-1-AP (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28983-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 28983-1-AP (same clone as 28983-1-PBS)

    IF Staining of NIH/3T3 using 28983-1-AP (same clone as 28983-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated NIH/3T3 cells using Phospho-RPS6KA1 (Ser380) antibody (28983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28983-1-PBS in a different storage buffer formulation.

Phospho-RPS6KA1 (Ser380) Polyclonal antibody, PBS Only

Phospho-RPS6KA1 (Ser380) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28983-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

p RPS6KA1, p RPS6KA1 (Ser380), p RPS6KA1 Ser380, Phospho RPS6KA1, Phospho RPS6KA1 (Ser380)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.

产品信息

28983-1-PBS targets Phospho-RPS6KA1 (Ser380) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 1
别名 p RPS6KA1, p RPS6KA1 (Ser380), p RPS6KA1 Ser380, Phospho RPS6KA1, Phospho RPS6KA1 (Ser380)
计算分子量 735 aa, 83 kDa
观测分子量83-90 kDa
GenBank蛋白编号BC014966
基因名称 RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2881237
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15418
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)