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Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal antibody

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C11D9

Cat no : 68041-1-Ig

Print datasheet

Synonyms

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

验证数据展示

  • WB analysis using 68041-1-Ig

    WB analysis using 68041-1-Ig

    Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control.

  • IF Staining of HeLa using 68041-1-Ig

    IF Staining of HeLa using 68041-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, λ phosphatase treated HeLa cells, λ phosphatase treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68041-1-Ig targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Synonyms HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC014966
Gene Symbol RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2918783
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

实验方案

Product Specific Protocols
WB protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 68041-1-IgDownload protocol
IF protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 68041-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols