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验证数据展示

  • WB analysis using 68041-1-Ig (same clone as 68041-1-PBS)

    WB analysis using 68041-1-Ig (same clone as 68041-1-PBS)

    Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control. This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68041-1-Ig (same clone as 68041-1-PBS)

    IF Staining of HeLa using 68041-1-Ig (same clone as 68041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal antibody, PBS Only

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68041-1-PBS

产品说明书

CloneNo. 2C11D9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IF/ICC, Indirect ELISA

别名

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.

产品信息

68041-1-PBS targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 1
别名 HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1
计算分子量 735 aa, 83 kDa
观测分子量80 kDa
GenBank蛋白编号BC014966
基因名称 RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2918783
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15418
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)