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验证数据展示

  • WB analysis of NIH/3T3 using 80575-3-RR

    WB analysis of NIH/3T3 using 80575-3-RR

    NIH/3T3 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

  • WB analysis of PC-12 using 80575-3-RR

    WB analysis of PC-12 using 80575-3-RR

    PC-12 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

  • WB analysis using 80575-3-RR

    WB analysis using 80575-3-RR

    HEK-293 cells treated with 10% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80575-3-RR

产品说明书

CloneNo. 242048C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Phos S6 Ribosomal protein (Ser240/244), Phos RPS6, Phos ribosomal protein S6, Phospho RPS6, Phospho-RPS6 (Ser240/244)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in20% fetal bovine serum after serum free NIH/3T3 cells, 10% fetal bovine serum after serum free HEK-293 cells, 20% fetal bovine serum after serum free PC-12 cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80575-3-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S6
别名 Phos S6 Ribosomal protein (Ser240/244), Phos RPS6, Phos ribosomal protein S6, Phospho RPS6, Phospho-RPS6 (Ser240/244)
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP62753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)

实验方案

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 80575-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols