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验证数据展示

  • FC experiment of HeLa using 81184-1-RR

    FC experiment of HeLa using 81184-1-RR

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone: 5J13, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA.

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 81184-1-RR
CloneNo. 5J13

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

p S6 Ribosomal protein, p RPS6 (Ser240/244), p RPS6, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected in100 nM Calyculin A (30 minutes) treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81184-1-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms p S6 Ribosomal protein, p RPS6 (Ser240/244), p RPS6, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244
Observed Molecular Weight32 kDa
GenBank Accession NumberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2935425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.