验证数据展示
经过测试的应用
Positive FC (Intra) detected in | 100 nM Calyculin A (30 minutes) treated HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81184-1-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ribosomal protein S6 |
Synonyms | p S6 Ribosomal protein, p RPS6 (Ser240/244), p RPS6, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244 |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | NM_001010 |
Gene Symbol | RPS6 |
Gene ID (NCBI) | 6194 |
RRID | AB_2935425 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P62753 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.