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验证数据展示

  • FC experiment of HEK-293 using 81184-1-RR

    FC experiment of HEK-293 using 81184-1-RR

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone:5J13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • WB analysis of HEK-293 using 81184-1-RR

    WB analysis of HEK-293 using 81184-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

  • IF Staining of HeLa using 81184-1-RR

    IF Staining of HeLa using 81184-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) antibody (81184-1-RR, Clone: 5J13 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 81184-1-RR

产品说明书

CloneNo. 5J13

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

p RPS6, p RPS6 (Ser240/244), p S6 Ribosomal protein, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells
Positive IF/ICC detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81184-1-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S6
别名 p RPS6, p RPS6 (Ser240/244), p S6 Ribosomal protein, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244
观测分子量32 kDa
GenBank蛋白编号NM_001010
基因名称 RPS6
Gene ID (NCBI) 6194
RRIDAB_2935425
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.

实验方案

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 81184-1-RRDownload protocol
IF protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 81184-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols