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Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5J13

Cat no : 81184-1-RR

Print datasheet

Synonyms

p RPS6 (Ser240/244), p RPS6, RPS6, S6, Phosphoprotein NP33

验证数据展示

  • IF Staining of HeLa using 81184-1-RR

    IF Staining of HeLa using 81184-1-RR

    Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) antibody (81184-1-RR, Clone: 5J13) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004).

  • WB analysis of HeLa using 81184-1-RR

    WB analysis of HeLa using 81184-1-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

  • WB analysis of HEK-293 using 81184-1-RR

    WB analysis of HEK-293 using 81184-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

  • FC experiment of HeLa using 81184-1-RR

    FC experiment of HeLa using 81184-1-RR

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone: 5J13, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA.

  • FC experiment of HEK-293 using 81184-1-RR

    FC experiment of HEK-293 using 81184-1-RR

    1x10^6 Calyculin A treated HEK-293 cells were intracellularly stained with 0.06 ug Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone:5J13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated HEK-293 cells
Positive IF/ICC detected in100 nM Calyculin A (30 minutes) treated HeLa cells
Positive FC (Intra) detected in100 nM Calyculin A (30 minutes) treated HeLa cells, Calyculin A treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81184-1-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms p RPS6 (Ser240/244), p RPS6, RPS6, S6, Phosphoprotein NP33
Observed Molecular Weight32 kDa
GenBank Accession NumberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.

实验方案

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 81184-1-RRDownload protocol
IF protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 81184-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols