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Phospho-SMAD2 (Ser465/467) Recombinant antibody, PBS Only

Phospho-SMAD2 (Ser465/467) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240826D11

Cat no : 80427-2-PBS

Print datasheet

Synonyms

SMAD2, JV18 1, JV18, hSMAD2, hMAD-2

验证数据展示

  • WB analysis using 80427-2-RR (same clone as 80427-2-PBS)

    WB analysis using 80427-2-RR (same clone as 80427-2-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80427-2-RR (Phospho-SMAD2 (Ser465/467) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80427-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMAD2 Ready-To-Use IHC Kit. SMAD2 primary antibody included.

产品信息

80427-2-PBS targets Phospho-SMAD2 (Ser465/467) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 2
Synonyms SMAD2, JV18 1, JV18, hSMAD2, hMAD-2
Calculated Molecular Weight 467 aa, 52 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC014840
Gene Symbol SMAD2
Gene ID (NCBI) 4087
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15796
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)