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验证数据展示

  • IF Staining of HeLa using 60479-1-Ig

    IF Staining of HeLa using 60479-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed untreated HeLa cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-STAT3 (Ser727) antibody (60479-1-Ig, Clone: 2G11G11) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 60479-1-Ig

    WB analysis using 60479-1-Ig

    UV treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • WB analysis using 60479-1-Ig

    WB analysis using 60479-1-Ig

    EGF treated and untreated A549 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • WB analysis using 60479-1-Ig

    WB analysis using 60479-1-Ig

    TNF alpha (HZ-1014) treated and untreated MCF-7 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • WB analysis using 60479-1-Ig

    WB analysis using 60479-1-Ig

    TNF alpha (HZ-1014) treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • WB analysis of HSC-T6 using 60479-1-Ig

    WB analysis of HSC-T6 using 60479-1-Ig

    UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (STAT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 60479-1-Ig

    FC experiment of HeLa using 60479-1-Ig

    1x10^6 untreated HeLa cells (dash lines), UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig, Clone: 2G11G11, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2, blue) was parallel stained as control. Cells were fixed with 4% PFA .

Phospho-STAT3 (Ser727) Monoclonal antibody

Phospho-STAT3 (Ser727) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 60479-1-Ig

产品说明书

CloneNo. 2G11G11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

p STAT3 (Ser727), p STAT3, p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, UV treated HSC-T6 cells, MCF-7 cells, NIH/3T3 cells, EGF treated A549 cells, UV treated HeLa cells, TNF alpha treated MCF-7 cells
Positive IF/ICC detected inUV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa cells
Positive FC (Intra) detected inUV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 signal transducer and activator of transcription 3 (acute-phase response factor)
别名 p STAT3 (Ser727), p STAT3, p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)
计算分子量 770 aa, 88 kDa
观测分子量85-90 kDa
GenBank蛋白编号BC000627
基因名称 STAT3
Gene ID (NCBI) 6774
RRIDAB_3670262
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgDownload protocol
IF protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols