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Phospho-STAT3 (Ser727) Monoclonal antibody, PBS Only

Phospho-STAT3 (Ser727) Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G11G11

Cat no : 60479-1-PBS

Print datasheet

Synonyms

STAT3, STAT 3, HIES, APRF, Acute-phase response factor

验证数据展示

  • IF Staining of HeLa using 60479-1-Ig (same clone as 60479-1-PBS)

    IF Staining of HeLa using 60479-1-Ig (same clone as 60479-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated HeLa cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-STAT3 (Ser727) antibody (60479-1-Ig, Clone: 2G11G11) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

  • WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    UV treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

  • WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    EGF treated and untreated A549 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

  • WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    TNF alpha (HZ-1014) treated and untreated MCF-7 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

  • WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

    TNF alpha (HZ-1014) treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 60479-1-Ig (same clone as 60479-1-PBS)

    WB analysis of HSC-T6 using 60479-1-Ig (same clone as 60479-1-PBS)

    UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (STAT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60479-1-Ig (same clone as 60479-1-PBS)

    FC experiment of HeLa using 60479-1-Ig (same clone as 60479-1-PBS)

    1x10^6 untreated HeLa cells (dash lines), UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig, Clone: 2G11G11, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

产品信息

60479-1-PBS targets Phospho-STAT3 (Ser727) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Synonyms STAT3, STAT 3, HIES, APRF, Acute-phase response factor
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight85-90 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40763
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.