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验证数据展示

  • WB analysis of HEK-293 using 81785-1-RR

    WB analysis of HEK-293 using 81785-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • FC experiment of HEK-293 using 81785-1-RR

    FC experiment of HEK-293 using 81785-1-RR

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.13 ug Phospho-TAK1 (Thr187) Recombinant antibody (81785-1-RR, Clone:2O6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TAK1 (Thr187) Recombinant antibody

Phospho-TAK1 (Thr187) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81785-1-RR

Print datasheet

CloneNo. 2O6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells, Calyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81785-1-RR targets Phospho-TAK1 (Thr187) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7
Synonyms p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)
Calculated Molecular Weight 579 aa, 64 kDa
Observed Molecular Weight75-85 kDa
GenBank Accession NumberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
RRIDAB_3085357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAK1 (Thr187) antibody 81785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols