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验证数据展示

  • WB analysis of HEK-293 using 81785-1-RR

    WB analysis of HEK-293 using 81785-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • FC experiment of HEK-293 using 81785-1-RR

    FC experiment of HEK-293 using 81785-1-RR

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.13 ug Phospho-TAK1 (Thr187) Recombinant antibody (81785-1-RR, Clone:2O6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TAK1 (Thr187) Recombinant antibody

Phospho-TAK1 (Thr187) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81785-1-RR

产品说明书

CloneNo. 2O6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells, Calyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81785-1-RR targets Phospho-TAK1 (Thr187) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 7
别名 p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)
计算分子量 579 aa, 64 kDa
观测分子量75-85 kDa
GenBank蛋白编号BC017715
基因名称 TAK1
Gene ID (NCBI) 6885
RRIDAB_3085357
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAK1 (Thr187) antibody 81785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols