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Phospho-TAK1 (Thr187) Recombinant antibody

Phospho-TAK1 (Thr187) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2O6

Cat no : 81785-1-RR

Print datasheet

Synonyms

MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), Phospho-TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a

验证数据展示

  • WB analysis of HEK-293 using 81785-1-RR

    WB analysis of HEK-293 using 81785-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells, Calyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81785-1-RR targets Phospho-TAK1 (Thr187) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7
Synonyms MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), Phospho-TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a
Calculated Molecular Weight 579 aa, 64 kDa
Observed Molecular Weight75-85 kDa
GenBank Accession NumberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAK1 (Thr187) antibody 81785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols