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验证数据展示

  • WB analysis using 82382-2-RR (same clone as 82382-2-PBS)

    WB analysis using 82382-2-RR (same clone as 82382-2-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82382-2-RR (Phospho-TBK1 (Ser172) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82382-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82382-2-RR (same clone as 82382-2-PBS)

    FC experiment of HeLa using 82382-2-RR (same clone as 82382-2-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-TBK1 (Ser172) Recombinant antibody (82382-2-RR, Clone:241787G12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82382-2-PBS in a different storage buffer formulation.

Phospho-TBK1 (Ser172) Recombinant antibody, PBS Only

Phospho-TBK1 (Ser172) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82382-2-PBS

产品说明书

CloneNo. 241787G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

p TBK1 (Ser172), Phospho TBK1 (Ser172), Phospho-NAK (Ser172), Phospho-TBK1, Phospho-TBK1-Ser172

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82382-2-PBS targets Phospho-TBK1 (Ser172) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TANK-binding kinase 1
别名 p TBK1 (Ser172), Phospho TBK1 (Ser172), Phospho-NAK (Ser172), Phospho-TBK1, Phospho-TBK1-Ser172
观测分子量84 kDa
GenBank蛋白编号BC034950
基因名称 TBK1
Gene ID (NCBI) 29110
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHD2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine/threonine protein kinase TBK1, discovered two decades ago as a member of the IκB kinase (IKK) protein kinase family, plays crucial roles in regulating innate immune response by promoting type I interferon (IFN) and modulating NF-κB signaling. The phosphorylation of Ser172 residue on TBK1 is critical and is commonly used as an indicator for its activity. (PMID: 39279883)