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Phospho-TSC2 (Ser939) Recombinant antibody, PBS Only

Phospho-TSC2 (Ser939) Recombinant Antibody for WB,Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B18

Cat No : 81654-1-PBS

Print datasheet

Synonyms

FLJ43106, LAM, Phospho-TSC2 (Ser939), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

验证数据展示

  • WB analysis of HEK-293 using 81654-1-RR (same clone as 81654-1-PBS)

    WB analysis of HEK-293 using 81654-1-RR (same clone as 81654-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

产品信息

81654-1-PBS targets Phospho-TSC2 (Ser939) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tuberous sclerosis 2
Synonyms FLJ43106, LAM, Phospho-TSC2 (Ser939), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).