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Phospho-TSC2 (Ser939) Recombinant antibody, PBS Only

Phospho-TSC2 (Ser939) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B18

Cat no : 81654-1-PBS

Print datasheet

Synonyms

Phospho TSC2 (Ser939), Phospho TSC2, p TSC2 Ser939, p TSC2 (Ser939), p TSC2

验证数据展示

  • WB analysis using 81654-1-RR (same clone as 81654-1-PBS)

    WB analysis using 81654-1-RR (same clone as 81654-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 81654-1-RR (same clone as 81654-1-PBS)

    FC experiment of HEK-293 using 81654-1-RR (same clone as 81654-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.06 ug Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR, Clone:2B18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

产品信息

81654-1-PBS targets Phospho-TSC2 (Ser939) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tuberous sclerosis 2
Synonyms Phospho TSC2 (Ser939), Phospho TSC2, p TSC2 Ser939, p TSC2 (Ser939), p TSC2
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).