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验证数据展示

  • WB analysis using 81654-1-RR

    WB analysis using 81654-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control.

  • FC experiment of HEK-293 using 81654-1-RR

    FC experiment of HEK-293 using 81654-1-RR

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.06 ug Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR, Clone:2B18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TSC2 (Ser939) Recombinant antibody

Phospho-TSC2 (Ser939) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81654-1-RR

产品说明书

CloneNo. 2B18

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Phospho TSC2 (Ser939), Phospho TSC2, p TSC2 Ser939, p TSC2 (Ser939), p TSC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells
Positive FC (Intra) detected inCalyculin A treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81654-1-RR targets Phospho-TSC2 (Ser939) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tuberous sclerosis 2
别名 Phospho TSC2 (Ser939), Phospho TSC2, p TSC2 Ser939, p TSC2 (Ser939), p TSC2
计算分子量 1807 aa, 201 kDa
观测分子量200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
RRIDAB_3085356
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49815
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).

实验方案

Product Specific Protocols
WB protocol for Phospho-TSC2 (Ser939) antibody 81654-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols