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验证数据展示

  • WB analysis of HEK-293 using 80698-1-RR (same clone as 80698-1-PBS)

    WB analysis of HEK-293 using 80698-1-RR (same clone as 80698-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80698-1-RR (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control. This data was developed using the same antibody clone with 80698-1-PBS in a different storage buffer formulation.

Phospho-TSC2 (Thr1462) Recombinant antibody, PBS Only

Phospho-TSC2 (Thr1462) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80698-1-PBS

产品说明书

CloneNo. 1L20

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, Indirect ELISA

别名

FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

产品信息

80698-1-PBS targets Phospho-TSC2 (Thr1462) in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tuberous sclerosis 2
别名 FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein
计算分子量 1807 aa, 201 kDa
观测分子量200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49815
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)