验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80698-1-RR targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tuberous sclerosis 2 |
| 别名 | FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein |
| 计算分子量 | 1807 aa, 201 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC150300 |
| 基因名称 | TSC2 |
| Gene ID (NCBI) | 7249 |
| RRID | AB_3085354 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49815 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TSC2 (Thr1462) antibody 80698-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |