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验证数据展示

  • WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

    WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

    Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 80218-1-RR (same clone as 80218-1-PBS)

    FC experiment of PC-3 using 80218-1-RR (same clone as 80218-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-ULK1 (Ser556) (80218-1-RR, Clone:2F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

Phospho-ULK1 (Ser556) Recombinant antibody, PBS Only

Phospho-ULK1 (Ser556) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80218-1-PBS

产品说明书

CloneNo. 2F5

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, Indirect ELISA

别名

ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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This antibody is equivalent to Ser555 in mice.

Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

产品信息

80218-1-PBS targets Phospho-ULK1 (Ser556) in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog
观测分子量 140 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
RRIDAB_2918877
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75385
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)