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Phospho-ULK1 (Ser556) Recombinant antibody, PBS Only

Phospho-ULK1 (Ser556) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F5

Cat no : 80218-1-PBS

Print datasheet

Synonyms

ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

验证数据展示

  • WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

    WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

    Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 80218-1-RR (same clone as 80218-1-PBS)

    FC experiment of PC-3 using 80218-1-RR (same clone as 80218-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-ULK1 (Ser556) (80218-1-RR, Clone:2F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

This antibody is equivalent to Ser555 in mice.

Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

产品信息

80218-1-PBS targets Phospho-ULK1 (Ser556) in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name unc-51-like kinase 1 (C. elegans)
Synonyms ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog
Observed Molecular Weight 140 kDa
GenBank Accession NumberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75385
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)