Phospho-ULK1 (Ser556) Recombinant antibody

Phospho-ULK1 (Ser556) Uni-rAbTM Recombinant Antibody for WB, ELISA, FC (Intra)
Cat No. 80218-1-RR

产品说明书

CloneNo. 2F5

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (3)

应用

WB, ELISA, FC (Intra) and More (1)

ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells
Positive FC (Intra) detected inCalyculin A treated PC-3 cells

This antibody is equivalent to Ser555 in mice.

Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80218-1-RR targets Phospho-ULK1 (Ser556) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse
文献引用反应性human, mouse, rat, goat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog
观测分子量 140 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
RRIDAB_2918877
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75385
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)

实验方案

Product Specific Protocols
WB protocol for Phospho-ULK1 (Ser556) antibody 80218-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Hazard Mater

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.

Authors - Feng Xiaoli
mouseWB

JCI Insight

ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing mRNA

Authors - Honglin Liu
humanWB

Sci Rep

EYA3 promotes the tumorigenesis of gastric cancer through activation of the mTORC1 signaling pathway and inhibition of autophagy

Authors - Zhen Xu
humanWB

Bioengineered

Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells.

Authors - Junli Zhang
humanWB

Oxid Med Cell Longev

tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOR

Authors - Zengfa Deng
mouseWB

Cell Death Dis

IKCa channels control breast cancer metabolism including AMPK-driven autophagy

Authors - Dominic Gross
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