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验证数据展示

  • WB analysis using 30737-1-AP

    WB analysis using 30737-1-AP

    Non-treated and HGF (HZ-1084) treated A549 cells were subjected to SDS PAGE followed by western blot with 30737-1-AP (Phospho-c-Met (Tyr1234/1235) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-c-Met (Tyr1234/1235) Polyclonal antibody

Phospho-c-Met (Tyr1234/1235) Polyclonal Antibody for WB, ELISA
Cat No. 30737-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

AUTS9, c Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHGF treated A549 cells
Planning an IHC experiment? We recommend our IHCeasy MET Ready-To-Use IHC Kit. MET primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

30737-1-AP targets Phospho-c-Met (Tyr1234/1235) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name met proto-oncogene (hepatocyte growth factor receptor)
Synonyms AUTS9, c Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met
Calculated Molecular Weight 1390 aa, 155 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC130420
Gene Symbol MET
Gene ID (NCBI) 4233
RRIDAB_3085352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Met (MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. The binding of HGF to MET induces MET clustering and phosphorylation of Y1234 and Y1235, followed by phosphorylation of Y1349 and Y1356 in the carboxyl terminal region, to which adaptor molecules associate and transmit signals downstream. (PMID: 28064454)

实验方案

Product Specific Protocols
WB protocol for Phospho-c-Met (Tyr1234/1235) antibody 30737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Ethnopharmacol

Anti-hepatocellular carcinoma activity of Sorbaria sorbifolia by regulating VEGFR and c-Met/apoptotic pathway

Authors - Zhao-Hua Xu
View Article
humanWB

Theranostics

Development of dual aptamers-functionalized c-MET PROTAC degraders for targeted therapy of osteosarcoma

Authors - Xuekun Fu
View Article