Phospho-c-Met (Tyr1234/1235) Polyclonal antibody
Phospho-c-Met (Tyr1234/1235) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HGF treated A549 cells |
Planning an IHC experiment? We recommend our IHCeasy MET Ready-To-Use IHC Kit. MET primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30737-1-AP targets Phospho-c-Met (Tyr1234/1235) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | met proto-oncogene (hepatocyte growth factor receptor) |
| Synonyms | AUTS9, c Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met |
| Calculated Molecular Weight | 1390 aa, 155 kDa |
| Observed Molecular Weight | 145 kDa |
| GenBank Accession Number | BC130420 |
| Gene Symbol | MET |
| Gene ID (NCBI) | 4233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08581 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Met (MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. The binding of HGF to MET induces MET clustering and phosphorylation of Y1234 and Y1235, followed by phosphorylation of Y1349 and Y1356 in the carboxyl terminal region, to which adaptor molecules associate and transmit signals downstream. (PMID: 28064454)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-c-Met (Tyr1234/1235) antibody 30737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Anti-hepatocellular carcinoma activity of Sorbaria sorbifolia by regulating VEGFR and c-Met/apoptotic pathway |