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验证数据展示

  • IF Staining of Y79 using 66232-1-Ig (same clone as 66232-1-PBS)

    IF Staining of Y79 using 66232-1-Ig (same clone as 66232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

  • WB analysis of human retina using 66232-1-Ig (same clone as 66232-1-PBS)

    WB analysis of human retina using 66232-1-Ig (same clone as 66232-1-PBS)

    human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

  • IF Staining of rat eye using 66232-1-Ig (same clone as 66232-1-PBS)

    IF Staining of rat eye using 66232-1-Ig (same clone as 66232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

Pikachurin Monoclonal antibody, PBS Only

Pikachurin Monoclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA
Cat No. 66232-1-PBS

产品说明书

CloneNo. 1C6D12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IF-P, Indirect ELISA

别名

EGFLAM, AGRNL, Agrin-like protein, AGRINL, Agrin like protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66232-1-PBS targets Pikachurin in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Pikachurin fusion protein Ag6362 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 EGF-like, fibronectin type III and laminin G domains
别名 EGFLAM, AGRNL, Agrin-like protein, AGRINL, Agrin like protein
计算分子量 111 kDa
观测分子量 111 kDa, 55-60 kDa
GenBank蛋白编号BC033177
基因名称 Pikachurin
Gene ID (NCBI) 133584
RRIDAB_2881623
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ63HQ2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).