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验证数据展示

  • IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Podocalyxin antibody included
  • IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Podocalyxin antibody included
  • IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

Podocalyxin Monoclonal antibody, PBS Only

Podocalyxin Monoclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 68250-1-PBS

产品说明书

CloneNo. 4A3C7

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IHC, IF-P, Indirect ELISA

别名

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Podocalyxin Ready-To-Use IHC Kit. Podocalyxin primary antibody included.

产品信息

68250-1-PBS targets Podocalyxin in IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human
免疫原 Podocalyxin fusion protein Ag12859 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 podocalyxin-like
别名 GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60
计算分子量 526 aa, 55 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC093730
基因名称 Podocalyxin
Gene ID (NCBI) 5420
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00592
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).