Podocalyxin Monoclonal antibody

Podocalyxin Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 68250-1-Ig

产品说明书

CloneNo. 4A3C7

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IHC, IF-P, ELISA

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy Podocalyxin Ready-To-Use IHC Kit. Podocalyxin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68250-1-Ig targets Podocalyxin in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human
免疫原 Podocalyxin fusion protein Ag12859 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 podocalyxin-like
别名 GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60
计算分子量 526 aa, 55 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC093730
基因名称 Podocalyxin
Gene ID (NCBI) 5420
RRIDAB_2935335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00592
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

实验方案

Product Specific Protocols
IHC protocol for Podocalyxin antibody 68250-1-IgDownload protocol
IF protocol for Podocalyxin antibody 68250-1-IgDownload protocol
FC protocol for Podocalyxin antibody 68250-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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