验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse small intestine tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67432-1-Ig targets Podoplanin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, IF-P, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | Podoplanin fusion protein Ag17691 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | podoplanin |
别名 | PDPN, PSEC0003, PA2.26 antigen, Aggrus, 29kDa cytosolic podoplanin intracellular domain |
计算分子量 | 238 aa, 25 kDa |
GenBank蛋白编号 | BC022812 |
基因名称 | Podoplanin |
Gene ID (NCBI) | 10630 |
RRID | AB_2882670 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q86YL7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for Podoplanin antibody 67432-1-Ig | Download protocol |
IF protocol for Podoplanin antibody 67432-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
CNS Neurosci Ther Exosome-transmitted podoplanin promotes tumor-associated macrophage-mediated immune tolerance in glioblastoma | ||
Am J Pathol SET domain-containing 5 (SETD5) facilitates tumor growth and pulmonary metastasis through up-regulation of AKT1 signaling in breast cancer. | ||
Cell Death Dis FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis. | ||
Drug Resist Updat PDPN positive CAFs contribute to HER2 positive breast cancer resistance to trastuzumab by inhibiting antibody-dependent NK cell-mediated cytotoxicity |