CoraLite®594-conjugated Podoplanin Monoclonal antibody

Podoplanin Monoclonal Antibody for FC
Cat No. CL594-67432

产品说明书

CloneNo. 1D9F3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -


经过测试的应用

Positive FC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy Podoplanin Ready-To-Use IHC Kit. Podoplanin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67432-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67432 targets Podoplanin in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Podoplanin fusion protein Ag17691 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 podoplanin
别名
计算分子量 238 aa, 25 kDa
GenBank蛋白编号BC022812
基因名称 Podoplanin
Gene ID (NCBI) 10630
RRIDAB_3673520
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86YL7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.

实验方案

Product Specific Protocols
FC protocol for CL594 Podoplanin antibody CL594-67432Download protocol
Standard Protocols
Click here to view our Standard Protocols
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