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  • Featured Product
  • KD/KO Validated

Prohibitin 2 Monoclonal antibody, PBS Only

Prohibitin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9C7

Cat no : 66424-1-PBS

Print datasheet

Synonyms

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

验证数据展示

  • WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

    WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66424-1-Ig (same clone as 66424-1-PBS)

    IF Staining of HepG2 using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

    WB analysis of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

    WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

    IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHB2 antibody included
  • IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

    IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHB2 antibody included
Planning an IHC experiment? We recommend our IHCeasy PHB2 Ready-To-Use IHC Kit. PHB2 primary antibody included.

产品信息

66424-1-PBS targets Prohibitin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Prohibitin 2 fusion protein Ag2977 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prohibitin 2
Synonyms BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC014766
Gene Symbol Prohibitin 2
Gene ID (NCBI) 11331
RRIDAB_2811041
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99623
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)