验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human breast cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66424-1-Ig targets Prohibitin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Prohibitin 2 fusion protein Ag2977 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | prohibitin 2 |
| 别名 | PHB2, 1D9C7, BAP, B-cell receptor-associated protein BAP37, D prohibitin |
| 计算分子量 | 299 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC014766 |
| 基因名称 | Prohibitin 2 |
| Gene ID (NCBI) | 11331 |
| RRID | AB_2811041 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99623 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Prohibitin 2 antibody 66424-1-Ig | Download protocol |
| IHC protocol for Prohibitin 2 antibody 66424-1-Ig | Download protocol |
| IF protocol for Prohibitin 2 antibody 66424-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells. | ||
Environ Pollut Fluoride induced mitochondrial impairment and PINK1-mediated mitophagy in Leydig cells of mice: In vivo and in vitro studies. | ||
Viruses Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection.
| ||
Mol Biol Cell Discovery of bactericides as an acute mitochondrial membrane damage inducer. | ||
PLoS One A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids. |
