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验证数据展示

  • IF Staining of HeLa using CL488-66424

    IF Staining of HeLa using CL488-66424

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66424 (Prohibitin 2 antibody) at dilution of 1:100.

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CoraLite® Plus 488-conjugated Prohibitin 2 Monoclonal antibody

Prohibitin 2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66424

产品说明书

CloneNo. 1D9C7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PHB2 Ready-To-Use IHC Kit. PHB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66424-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66424 targets Prohibitin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 Prohibitin 2 fusion protein Ag2977 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prohibitin 2
别名 BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA
计算分子量 299 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC014766
基因名称 Prohibitin 2
Gene ID (NCBI) 11331
RRIDAB_2883335
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99623
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Prohibitin 2 antibody CL488-66424Download protocol
Standard Protocols
Click here to view our Standard Protocols