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验证数据展示

  • WB analysis using 18571-1-AP

    WB analysis using 18571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 18571-1-AP

    WB analysis using 18571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

QPCT Polyclonal antibody

QPCT Polyclonal Antibody for WB, ELISA
Cat No. 18571-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC, GCT, Glutaminyl cyclase, Glutamyl cyclase, QC, QPCT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaki-2 cells, human urine sample

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18571-1-AP targets QPCT in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 QPCT fusion protein Ag13416 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaminyl-peptide cyclotransferase
别名 EC, GCT, Glutaminyl cyclase, Glutamyl cyclase, QC, QPCT
计算分子量 361 aa, 41 kDa
观测分子量41 kDa, 35 kDa
GenBank蛋白编号BC047756
基因名称 QPCT
Gene ID (NCBI) 25797
RRIDAB_3669301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutaminyl-peptide cyclotransferase (QPCT) gene encodes glutaminyl cyclase (QC), an enzyme that plays a role in post-translational modification of proteins by converting N-terminal glutamate to pyroglutamine. QPCT plays a specific role in promoting tumor cell proliferation, migration and angiogenesis as well as in inhibiting tumor cell apoptosis in cancers such as thyroid cancer and renal cell carcinoma (PMID: 38417050). Human QPCT has two isoforms produced by alternative splicing, 41 kDa and 35 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for QPCT antibody 18571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols