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  • Featured Product
  • KD/KO Validated

QPRT Polyclonal antibody

QPRT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25174-1-AP

Print datasheet

Synonyms

QAPRTase, QPRT, QPRTase

验证数据展示

  • WB analysis using 25174-1-AP

    WB analysis using 25174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25174-1-AP

    IF Staining of HepG2 using 25174-1-AP

    Immunofluorescent analysis of HepG2 cells using 25174-1-AP (QPRT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25174-1-AP targets QPRT in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen QPRT fusion protein Ag17680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name quinolinate phosphoribosyltransferase
Synonyms QAPRTase, QPRT, QPRTase
Calculated Molecular Weight 297 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018910
Gene Symbol QPRT
Gene ID (NCBI) 23475
RRIDAB_2879941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15274
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

实验方案

Product Specific Protocols
WB protocol for QPRT antibody 25174-1-APDownload protocol
IF protocol for QPRT antibody 25174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Genet

Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney.

Authors - Xiao Y Zhou
View Article
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
View Article
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin
View Article
mouseIF

Aging Cell

Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging

Authors - Qingling Yang
View Article
human,mouseWB

Nat Commun

Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas

Authors - Jianxing Yin
  • KD Validated
View Article