验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25174-1-AP targets QPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | QPRT fusion protein Ag17680 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | quinolinate phosphoribosyltransferase |
别名 | QAPRTase, QPRT, QPRTase |
计算分子量 | 297 aa, 31 kDa |
观测分子量 | 31 kDa |
GenBank蛋白编号 | BC018910 |
基因名称 | QPRT |
Gene ID (NCBI) | 23475 |
RRID | AB_2879941 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q15274 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for QPRT antibody 25174-1-AP | Download protocol |
IF protocol for QPRT antibody 25174-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Genet Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney. | ||
J Immunol Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues | ||
J Biol Chem Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity | ||
Nat Commun Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas
| ||
Front Endocrinol (Lausanne) Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data |