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验证数据展示

  • IF Staining of HepG2 using CL488-25174

    IF Staining of HepG2 using CL488-25174

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 QPRT antibody (CL488-25174) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-25174

    FC experiment of HepG2 using CL488-25174

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated QPRT Polyclonal antibody (CL488-25174)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated QPRT Polyclonal antibody

QPRT Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25174

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

QPRTase, QAPRTase, Nicotinate-nucleotide pyrophosphorylase [carboxylating], EC:2.4.2.19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 25174-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25174 targets QPRT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen QPRT fusion protein Ag17680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name quinolinate phosphoribosyltransferase
Synonyms QPRTase, QAPRTase, Nicotinate-nucleotide pyrophosphorylase [carboxylating], EC:2.4.2.19
Calculated Molecular Weight 297 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018910
Gene Symbol QPRT
Gene ID (NCBI) 23475
RRIDAB_3672787
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15274
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 QPRT antibody CL488-25174Download protocol
FC protocol for CL Plus 488 QPRT antibody CL488-25174Download protocol
Standard Protocols
Click here to view our Standard Protocols