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验证数据展示

  • FC experiment of HeLa using CL488-67902

    FC experiment of HeLa using CL488-67902

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB11A (CL488-67902, Clone:4A4C9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RAB11A Monoclonal antibody

RAB11A Monoclonal Antibody for FC (Intra)
Cat No. CL488-67902

产品说明书

CloneNo. 4A4C9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB11A/B Ready-To-Use IHC Kit. RAB11A/B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67902-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67902 targets RAB11A in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 RAB11A fusion protein Ag8879 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB11A, member RAS oncogene family
别名 RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2
计算分子量 216 aa, 24 kDa
观测分子量24 kDa
GenBank蛋白编号BC013348
基因名称 RAB11A
Gene ID (NCBI) 8766
RRIDAB_2923822
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP62491
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RAB11A antibody CL488-67902Download protocol
Standard Protocols
Click here to view our Standard Protocols