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验证数据展示

  • FC experiment of HeLa using CL488-67902

    FC experiment of HeLa using CL488-67902

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB11A (CL488-67902, Clone:4A4C9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RAB11A Monoclonal antibody

RAB11A Monoclonal Antibody for FC (Intra)
Cat No. CL488-67902

Print datasheet

CloneNo. 4A4C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

库存：1-2周货期

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB11A/B Ready-To-Use IHC Kit. RAB11A/B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67902-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67902 targets RAB11A in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen RAB11A fusion protein Ag8879 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB11A, member RAS oncogene family
Synonyms RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC013348
Gene Symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2923822
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP62491
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RAB11A antibody CL488-67902Download protocol
Standard Protocols
Click here to view our Standard Protocols