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  • Featured Product
  • KD/KO Validated

RAB1A Polyclonal antibody

RAB1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11671-1-AP

Print datasheet

Synonyms

YPT1 related protein, YPT1, Ras-related protein Rab-1A, Ras related protein Rab 1A, RAB1

验证数据展示

  • WB analysis using 11671-1-AP

    WB analysis using 11671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11671-1-AP

    WB analysis using 11671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

  • IHC staining of human lung cancer using 11671-1-AP

    IHC staining of human lung cancer using 11671-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1A antibody included
  • IHC staining of human lung cancer using 11671-1-AP

    IHC staining of human lung cancer using 11671-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1A antibody included
  • IF Staining of NIH/3T3 using 11671-1-AP

    IF Staining of NIH/3T3 using 11671-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of C2C12 using 11671-1-AP

    IF Staining of C2C12 using 11671-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using RAB1A antibody (11671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of A549 using 11671-1-AP

    IF Staining of A549 using 11671-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of A549 using 11671-1-AP

    IP experiment of A549 using 11671-1-AP

    IP result of anti-RAB1A (IP:11671-1-AP, 4ug; Detection:11671-1-AP 1:4000) with A549 cells lysate 920 ug.

  • IP experiment of C2C12 using 11671-1-AP

    IP experiment of C2C12 using 11671-1-AP

    IP result of anti-RAB1A (IP:11671-1-AP, 4ug; Detection:11671-1-AP 1:4000) with C2C12 cells lysate 1280 ug.

  • FC experiment of C2C12 using 11671-1-AP

    FC experiment of C2C12 using 11671-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human RAB1A (11671-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 11671-1-AP

    WB analysis using 11671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, NIH/3T3 cells, U-251 cells, rat brain tissue, U2OS cells
Positive IP detected inA549 cells, C2C12 cells
Positive IHC detected inhuman lung cancer tissue, human liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, NIH/3T3 cells, A549 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1A Ready-To-Use IHC Kit. RAB1A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11671-1-AP targets RAB1A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen RAB1A fusion protein Ag2264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB1A, member RAS oncogene family
Synonyms YPT1 related protein, YPT1, Ras-related protein Rab-1A, Ras related protein Rab 1A, RAB1
Calculated Molecular Weight 205 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC000905
Gene Symbol RAB1A
Gene ID (NCBI) 5861
RRIDAB_2173437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62820
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

实验方案

Product Specific Protocols
WB protocol for RAB1A antibody 11671-1-APDownload protocol
IHC protocol for RAB1A antibody 11671-1-APDownload protocol
IF protocol for RAB1A antibody 11671-1-APDownload protocol
IP protocol for RAB1A antibody 11671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IP

Cancer Cell

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

Authors - Janice D Thomas
  • KD Validated
View Article
humanIP

EMBO J

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

Authors - Chantal Sellier
View Article
mouseWB

Hum Mol Genet

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Authors - Gaynor A Smith
View Article
humanWB,IHC,IF

Cancer Lett

Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer.

Authors - Tinglei Huang
  • KD Validated
View Article
mouseWB

Mol Cell Proteomics

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Authors - Campbell-Valois François-Xavier FX
View Article
mouseIF

Hum Mol Genet

Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.

Authors - Mukesh Gautam
View Article