验证数据展示
经过测试的应用
| Positive IF/ICC detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy RAB1A Ready-To-Use IHC Kit. RAB1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11671-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11671 targets RAB1A in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RAB1A fusion protein Ag2264 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB1A, member RAS oncogene family |
| 别名 | DKFZp564B163, RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein |
| 计算分子量 | 205 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC000905 |
| 基因名称 | RAB1A |
| Gene ID (NCBI) | 5861 |
| RRID | AB_3672504 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62820 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RAB1A antibody CL488-11671 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |