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RAB1B Recombinant antibody, PBS Only

RAB1B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240635D9

Cat no : 83687-4-PBS

Print datasheet

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 240635D9

验证数据展示

  • WB analysis using 83687-4-RR (same clone as 83687-4-PBS)

    WB analysis using 83687-4-RR (same clone as 83687-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83687-4-RR (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83687-4-RR (same clone as 83687-4-PBS)

    FC experiment of U-251 using 83687-4-RR (same clone as 83687-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug RAB1B Recombinant antibody (83687-4-RR, Clone:240635D9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83687-4-RR (same clone as 83687-4-PBS)

    FC experiment of HeLa using 83687-4-RR (same clone as 83687-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB1B Recombinant antibody (83687-4-RR, Clone:240635D9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83687-4-RR

    Affinity and Kinetic Characterization of 83687-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83687-4-RR against Human RAB1B were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.

产品信息

83687-4-PBS targets RAB1B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB1B fusion protein Ag12197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB1B, member RAS oncogene family
Synonyms Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 240635D9
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0U4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport, was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets. (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).