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RAB1B Recombinant antibody

RAB1B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240635D9

Cat no : 83687-4-RR

Print datasheet

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 240635D9

验证数据展示

  • WB analysis using 83687-4-RR

    WB analysis using 83687-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83687-4-RR (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-251 using 83687-4-RR

    FC experiment of U-251 using 83687-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug RAB1B Recombinant antibody (83687-4-RR, Clone:240635D9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 83687-4-RR

    FC experiment of HeLa using 83687-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB1B Recombinant antibody (83687-4-RR, Clone:240635D9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83687-4-RR

    Affinity and Kinetic Characterization of 83687-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83687-4-RR against Human RAB1B were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, CHO cells, A375 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive FC (Intra) detected inU-251 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83687-4-RR is RAB1B Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB1B fusion protein Ag12197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB1B, member RAS oncogene family
Synonyms Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 240635D9
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport, was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets. (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).

实验方案

Product Specific Protocols
WB protocol for RAB1B antibody 83687-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols