• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RAB25 Polyclonal antibody

RAB25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13189-1-AP

Print datasheet

Synonyms

CATX 8, CATX8, RAB25, Ras related protein Rab 25

验证数据展示

  • WB analysis of HT-29 using 13189-1-AP

    WB analysis of HT-29 using 13189-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 13189-1-AP

    IHC staining of human renal cell carcinoma using 13189-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 13189-1-AP

    IHC staining of human renal cell carcinoma using 13189-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHT-29 cells, Caco-2 cells, T-47D cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen RAB25 fusion protein Ag3864 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB25, member RAS oncogene family
Synonyms CATX 8, CATX8, RAB25, Ras related protein Rab 25
Calculated Molecular Weight 213 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC033322
Gene Symbol RAB25
Gene ID (NCBI) 57111
RRIDAB_2877921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB25 antibody 13189-1-APDownload protocol
IHC protocol for RAB25 antibody 13189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Transl Cancer Res

Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma

Authors - Tianyu Zheng
View Article