- Featured Product
- KD/KO Validated
RAB27B Polyclonal antibody
RAB27B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | rat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells |
Positive IHC detected in | human pancreas cancer tissue, human oesophagus cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human prostate cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U-87 MG cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13412-1-AP targets RAB27B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine |
Immunogen | RAB27B fusion protein Ag4064 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RAB27B, member RAS oncogene family |
Synonyms | C25KG, RAB27B, Ras related protein Rab 27B |
Calculated Molecular Weight | 218 aa, 25 kDa |
Observed Molecular Weight | 25-30 kDa |
GenBank Accession Number | BC027474 |
Gene Symbol | RAB27B |
Gene ID (NCBI) | 5874 |
RRID | AB_2176732 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00194 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RAB27B antibody 13412-1-AP | Download protocol |
IHC protocol for RAB27B antibody 13412-1-AP | Download protocol |
IF protocol for RAB27B antibody 13412-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. | ||
Cell Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways. | ||
Immunity MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation. | ||
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. | ||