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验证数据展示

  • FC experiment of HeLa using CL647-13412

    FC experiment of HeLa using CL647-13412

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human RAB27B (CL647-13412) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated RAB27B Polyclonal antibody

RAB27B Polyclonal Antibody for FC (Intra)
Cat No. CL647-13412

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 13412-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13412 targets RAB27B in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen RAB27B fusion protein Ag4064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB27B, member RAS oncogene family
Synonyms C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2
Calculated Molecular Weight 218 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC027474
Gene Symbol RAB27B
Gene ID (NCBI) 5874
RRIDAB_3673569
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00194
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 RAB27B antibody CL647-13412Download protocol
Standard Protocols
Click here to view our Standard Protocols