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验证数据展示

  • FC experiment of HeLa using CL647-13412

    FC experiment of HeLa using CL647-13412

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human RAB27B (CL647-13412) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated RAB27B Polyclonal antibody

RAB27B Polyclonal Antibody for FC (Intra)
Cat No. CL647-13412

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 13412-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13412 targets RAB27B in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 RAB27B fusion protein Ag4064 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB27B, member RAS oncogene family
别名 C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2
计算分子量 218 aa, 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC027474
基因名称 RAB27B
Gene ID (NCBI) 5874
RRIDAB_3673569
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00194
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 RAB27B antibody CL647-13412Download protocol
Standard Protocols
Click here to view our Standard Protocols