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RAB2A Monoclonal antibody

RAB2A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3E1B10

Cat no : 67501-1-Ig

Print datasheet

Synonyms

RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10

验证数据展示

  • WB analysis using 67501-1-Ig

    WB analysis using 67501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67501-1-Ig (RAB2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67501-1-Ig

    IHC staining of mouse brain using 67501-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB2A antibody included
  • IHC staining of mouse brain using 67501-1-Ig

    IHC staining of mouse brain using 67501-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB2A antibody included
  • IF Staining of U2OS using 67501-1-Ig

    IF Staining of U2OS using 67501-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67501-1-Ig

    IF Staining of mouse brain using 67501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67501-1-Ig

    IF Staining of mouse brain using 67501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67501-1-Ig

    IF Staining of HeLa using 67501-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inU2OS cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inU2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB2A Ready-To-Use IHC Kit. RAB2A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67501-1-Ig targets RAB2A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RAB2A fusion protein Ag7939 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RAB2A, member RAS oncogene family
Synonyms RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10
Calculated Molecular Weight24 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC008929
Gene Symbol RAB2A
Gene ID (NCBI) 5862
RRIDAB_2882725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61019
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B.

实验方案

Product Specific Protocols
WB protocol for RAB2A antibody 67501-1-IgDownload protocol
IHC protocol for RAB2A antibody 67501-1-IgDownload protocol
IF protocol for RAB2A antibody 67501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Front Physiol

Upregulation of Amy1 in the salivary glands of mice exposed to a lunar gravity environment using the multiple artificial gravity research system

Authors - Takehito Ouchi
View Article