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验证数据展示

  • WB analysis using 67501-1-Ig

    WB analysis using 67501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67501-1-Ig (RAB2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67501-1-Ig

    IHC staining of mouse brain using 67501-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB2A antibody included
  • IHC staining of mouse brain using 67501-1-Ig

    IHC staining of mouse brain using 67501-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB2A antibody included
  • IF Staining of U2OS using 67501-1-Ig

    IF Staining of U2OS using 67501-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67501-1-Ig

    IF Staining of mouse brain using 67501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67501-1-Ig

    IF Staining of mouse brain using 67501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67501-1-Ig

    IF Staining of HeLa using 67501-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RAB2A Monoclonal antibody

RAB2A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67501-1-Ig

产品说明书

CloneNo. 3E1B10
引用文献(2)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inU2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB2A Ready-To-Use IHC Kit. RAB2A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67501-1-Ig targets RAB2A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RAB2A fusion protein Ag7939 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB2A, member RAS oncogene family
别名 RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10
计算分子量24 kDa
观测分子量 21 kDa
GenBank蛋白编号BC008929
基因名称 RAB2A
Gene ID (NCBI) 5862
RRIDAB_2882725
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61019
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B.

实验方案

Product Specific Protocols
WB protocol for RAB2A antibody 67501-1-IgDownload protocol
IHC protocol for RAB2A antibody 67501-1-IgDownload protocol
IF protocol for RAB2A antibody 67501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

EMBO J

Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes

Authors - Min Xu
View Article
mouseIF

Front Physiol

Upregulation of Amy1 in the salivary glands of mice exposed to a lunar gravity environment using the multiple artificial gravity research system

Authors - Takehito Ouchi
View Article