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CoraLite®594-conjugated RAB2A Monoclonal antibody

RAB2A Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E1B10

Cat no : CL594-67501

Print datasheet

Synonyms

RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10

验证数据展示

  • IF Staining of mouse brain using CL594-67501

    IF Staining of mouse brain using CL594-67501

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 RAB2A antibody (CL594-67501, Clone: 3E1B10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67501

    FC experiment of HeLa using CL594-67501

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB2A (CL594-67501, Clone:3E1B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB2A Ready-To-Use IHC Kit. RAB2A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67501-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67501 targets RAB2A in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB2A fusion protein Ag7939 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RAB2A, member RAS oncogene family
Synonyms RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10
Calculated Molecular Weight24 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC008929
Gene Symbol RAB2A
Gene ID (NCBI) 5862
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61019
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB2A antibody CL594-67501Download protocol
FC protocol for CL594 RAB2A antibody CL594-67501Download protocol
Standard Protocols
Click here to view our Standard Protocols