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RAB32 Recombinant antibody, PBS Only

RAB32 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230315A9

Cat no : 82960-1-PBS

Print datasheet

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32, 230315A9

验证数据展示

  • WB analysis using 82960-1-RR (same clone as 82960-1-PBS)

    WB analysis using 82960-1-RR (same clone as 82960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82960-1-RR (RAB32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82960-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82960-1-RR (same clone as 82960-1-PBS)

    IF Staining of HeLa using 82960-1-RR (same clone as 82960-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-1-RR, Clone: 230315A9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82960-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 82960-1-RR (same clone as 82960-1-PBS)

    FC experiment of U-251 using 82960-1-RR (same clone as 82960-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human RAB32 (82960-1-RR, Clone:230315A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82960-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82960-1-RR

    Affinity and Kinetic Characterization of 82960-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82960-1-RR against Human RAB32 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.

产品信息

82960-1-PBS targets RAB32 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen RAB32 fusion protein Ag1450 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB32, member RAS oncogene family
Synonyms Ras-related protein Rab-32, Ras related protein Rab 32, 230315A9
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC015061
Gene Symbol RAB32
Gene ID (NCBI) 10981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.