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CoraLite® Plus 488-conjugated RAB33A Monoclonal antibody

RAB33A Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C7F11

Cat no : CL488-68389

Print datasheet

Synonyms

验证数据展示

  • IF Staining of PC-12 using CL488-68389

    IF Staining of PC-12 using CL488-68389

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 RAB33A antibody (CL488-68389, Clone: 1C7F11 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 68389-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68389 targets RAB33A in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen RAB33A fusion protein Ag33188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB33A, member RAS oncogene family
Synonyms
Calculated Molecular Weight 27 kDa
GenBank Accession NumberBC009996
Gene Symbol RAB33A
Gene ID (NCBI) 9363
RRIDAB_3673001
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14088
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB33A antibody CL488-68389Download protocol
Standard Protocols
Click here to view our Standard Protocols