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验证数据展示

  • WB analysis using 83740-4-RR

    WB analysis using 83740-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83740-4-RR (RAB37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 83740-4-RR

    IP experiment of mouse brain using 83740-4-RR

    IP result of anti-RAB37 (IP:83740-4-RR, 4ug; Detection:83740-4-RR 1:2000) with mouse brain tissue lysate 1840 ug.

  • Affinity and Kinetic Characterization of 83740-4-RR

    Affinity and Kinetic Characterization of 83740-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83740-4-RR against Human RAB37 were performed. The affinity constant is 1.25 nM.

RAB37 Recombinant antibody

RAB37 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 83740-4-RR

产品说明书

CloneNo. 240838D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Ras-related protein Rab-37, Ras related protein Rab 37, 240838D6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A549 cells, mouse brain tissues, rat brain tissues
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83740-4-RR targets RAB37 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAB37 fusion protein Ag3717 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB37, member RAS oncogene family
别名 Ras-related protein Rab-37, Ras related protein Rab 37, 240838D6
计算分子量 223 aa, 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC016615
基因名称 RAB37
Gene ID (NCBI) 326624
RRIDAB_3671338
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96AX2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.

实验方案

Product Specific Protocols
WB protocol for RAB37 antibody 83740-4-RRDownload protocol
IP protocol for RAB37 antibody 83740-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols