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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of SH-SY5Y using CL488-68014

    FC experiment of SH-SY5Y using CL488-68014

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB39B (CL488-68014, Clone:2F4F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RAB39B Monoclonal antibody

RAB39B Monoclonal Antibody for FC (Intra)
Cat No. CL488-68014

产品说明书

CloneNo. 2F4F7

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

FC (Intra)

别名

Ras-related protein Rab-39B, Ras related protein Rab 39B, 2F4F7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488

规格: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 68014-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68014 targets RAB39B in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 RAB39B fusion protein Ag27170 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB39B, member RAS oncogene family
别名 Ras-related protein Rab-39B, Ras related protein Rab 39B, 2F4F7
计算分子量 213 aa, 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC009714
基因名称 RAB39B
Gene ID (NCBI) 116442
RRIDAB_2923859
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96DA2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB39B is a member of the RAB GTPase family with a putative role in vesicle trafficking. RAB39B is predominantly expressed in neuronal cells and is localized to the endoplasmic reticulum, Golgi and trans‐Golgi recycling endosomes. Several reports suggested that RAB39B may regulate protein trafficking and the PI3K-AKT-mTOR pathway. Mutations in RAB39B are known to be associated with X-linked intellectual disability (XLID), Parkinson's disease, and autism.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RAB39B antibody CL488-68014Download protocol
Standard Protocols
Click here to view our Standard Protocols