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RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A3A1

Cat No : 66688-1-Ig

Print datasheet

Synonyms

DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

验证数据展示

  • WB analysis using 66688-1-Ig

    WB analysis using 66688-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66688-1-Ig

    IHC staining of mouse brain using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66688-1-Ig

    IHC staining of mouse brain using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 66688-1-Ig

    WB analysis of HeLa using 66688-1-Ig

    WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells.

  • IF Staining of HEK-293 using 66688-1-Ig

    IF Staining of HEK-293 using 66688-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66688-1-Ig targets RAB3GAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen RAB3GAP1 fusion protein Ag17710 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB3 GTPase activating protein subunit 1 (catalytic)
Synonyms DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1
Calculated Molecular Weight 981 aa, 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC146809
Gene Symbol RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_2882042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
IHC protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
IF protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols