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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A3A1

Cat no : CL488-66688

Print datasheet

Synonyms

RAB3 GTPase-activating protein 130 kDa subunit, Rab3 GAP p130, Rab3 GAP, P130, KIAA0066

验证数据展示

  • IF Staining of HEK-293 using CL488-66688

    IF Staining of HEK-293 using CL488-66688

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 RAB3GAP1 antibody (CL488-66688, Clone: 4A3A1 ) at dilution of 1:100.

  • FC experiment of HEK-293 using CL488-66688

    FC experiment of HEK-293 using CL488-66688

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB3GAP1 (CL488-66688, Clone:4A3A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66688-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66688 targets RAB3GAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB3GAP1 fusion protein Ag17710 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB3 GTPase activating protein subunit 1 (catalytic)
Synonyms RAB3 GTPase-activating protein 130 kDa subunit, Rab3 GAP p130, Rab3 GAP, P130, KIAA0066
Calculated Molecular Weight 981 aa, 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC146809
Gene Symbol RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_2919358
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15042
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB3GAP1 antibody CL488-66688Download protocol
FC protocol for CL Plus 488 RAB3GAP1 antibody CL488-66688Download protocol
Standard Protocols
Click here to view our Standard Protocols