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验证数据展示

  • WB analysis using 29818-1-AP

    WB analysis using 29818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29818-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 29818-1-AP

    IP experiment of Jurkat using 29818-1-AP

    IP result of anti-RAB3IP/Rabin8 (IP:29818-1-AP, 4ug; Detection:29818-1-AP 1:4000) with Jurkat cells lysate 1320 ug.

RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for WB, IP, ELISA
Cat No. 29818-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29818-1-AP targets RAB3IP/Rabin8 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 RAB3IP/Rabin8 fusion protein Ag31291 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3A interacting protein (rabin3)
别名 RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein
计算分子量 476 aa, 53 kDa
观测分子量 51 kDa
GenBank蛋白编号BC015548
基因名称 RAB3IP
Gene ID (NCBI) 117177
RRIDAB_3669697
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.

实验方案

Product Specific Protocols
WB protocol for RAB3IP/Rabin8 antibody 29818-1-APDownload protocol
IP protocol for RAB3IP/Rabin8 antibody 29818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols