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RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 29818-1-AP

Print datasheet

Synonyms

RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

验证数据展示

  • WB analysis using 29818-1-AP

    WB analysis using 29818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29818-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 29818-1-AP

    IP experiment of Jurkat using 29818-1-AP

    IP result of anti-RAB3IP/Rabin8 (IP:29818-1-AP, 4ug; Detection:29818-1-AP 1:4000) with Jurkat cells lysate 1320 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29818-1-AP targets RAB3IP/Rabin8 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen RAB3IP/Rabin8 fusion protein Ag31291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB3A interacting protein (rabin3)
Synonyms RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC015548
Gene Symbol RAB3IP
Gene ID (NCBI) 117177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.

实验方案

Product Specific Protocols
WB protocol for RAB3IP/Rabin8 antibody 29818-1-APDownload protocol
IP protocol for RAB3IP/Rabin8 antibody 29818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols