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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-12321

Print datasheet

Synonyms

FLJ14660, FLJ22548, Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, Rabin 3, RABIN3, rabin8, SSX2 interacting protein

验证数据展示

  • IF Staining of HepG2 using CL594-12321

    IF Staining of HepG2 using CL594-12321

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RAB3IP/Rabin8 antibody (CL594-12321) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12321-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-12321 targets RAB3IP/Rabin8 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen RAB3IP/Rabin8 fusion protein Ag2974 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB3A interacting protein (rabin3)
Synonyms FLJ14660, FLJ22548, Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, Rabin 3, RABIN3, rabin8, SSX2 interacting protein
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC015548
Gene Symbol RAB3IP
Gene ID (NCBI) 117177
RRIDAB_2919782
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QF0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB3IP/Rabin8 antibody CL594-12321Download protocol
Standard Protocols
Click here to view our Standard Protocols