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验证数据展示

  • IF Staining of HepG2 using CL594-12321

    IF Staining of HepG2 using CL594-12321

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RAB3IP/Rabin8 antibody (CL594-12321) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for IF/ICC
Cat No. CL594-12321

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

FLJ14660, FLJ22548, Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, Rabin 3, RABIN3, rabin8, SSX2 interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12321-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-12321 targets RAB3IP/Rabin8 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 RAB3IP/Rabin8 fusion protein Ag2974 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3A interacting protein (rabin3)
别名 FLJ14660, FLJ22548, Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, Rabin 3, RABIN3, rabin8, SSX2 interacting protein
计算分子量 476 aa, 53 kDa
观测分子量 56 kDa
GenBank蛋白编号BC015548
基因名称 RAB3IP
Gene ID (NCBI) 117177
RRIDAB_2919782
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QF0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB3IP/Rabin8 antibody CL594-12321Download protocol
Standard Protocols
Click here to view our Standard Protocols