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验证数据展示

  • WB analysis using 83949-4-RR

    WB analysis using 83949-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83949-4-RR

    Affinity and Kinetic Characterization of 83949-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83949-4-RR against Human RAB5A were performed. The affinity constant is 26.7 nM.

RAB5A Recombinant antibody

RAB5A Recombinant Antibody for WB, ELISA
Cat No. 83949-4-RR

产品说明书

CloneNo. 241041D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

RAB5, Ras-related protein Rab-5A, Ras related protein Rab 5A, EC:3.6.5.2, 241041D7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tisue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83949-4-RR targets RAB5A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAB5A fusion protein Ag2549 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB5A, member RAS oncogene family
别名 RAB5, Ras-related protein Rab-5A, Ras related protein Rab 5A, EC:3.6.5.2, 241041D7
计算分子量 215 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC001267
基因名称 RAB5A
Gene ID (NCBI) 5868
RRIDAB_3671528
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP20339
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

实验方案

Product Specific Protocols
WB protocol for RAB5A antibody 83949-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols