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CoraLite® Plus 488-conjugated RAB5A Monoclonal antibody

RAB5A Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6A5

Cat no : CL488-66339

Print datasheet

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

验证数据展示

  • IF Staining of HeLa using CL488-66339

    IF Staining of HeLa using CL488-66339

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAB5A antibody (CL488-66339, Clone: 1B6A5 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66339-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66339 targets RAB5A in IF/ICC applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, pig
Immunogen RAB5A fusion protein Ag16781 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RAB5A, member RAS oncogene family
Synonyms RAB5, RAB5A, Ras related protein Rab 5A
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_2883313
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20339
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion. This anitbody is CL488 conjugated.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB5A antibody CL488-66339Download protocol
Standard Protocols
Click here to view our Standard Protocols