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  • Featured Product
  • KD/KO Validated

RAB5B Polyclonal antibody

RAB5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27403-1-AP

Print datasheet

Synonyms

Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2

验证数据展示

  • WB analysis of HeLa using 27403-1-AP

    WB analysis of HeLa using 27403-1-AP

    WB result of RAB5B antibody (27403-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB5B transfected HeLa cells.

  • WB analysis of mouse lung using 27403-1-AP

    WB analysis of mouse lung using 27403-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 27403-1-AP

    IF Staining of NIH/3T3 using 27403-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 27403-1-AP (RAB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 27403-1-AP

    IHC staining of human colon using 27403-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27403-1-AP (RAB5B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human stomach using 27403-1-AP

    IHC staining of human stomach using 27403-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27403-1-AP (RAB5B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue, Jurkat cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27403-1-AP targets RAB5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RAB5B fusion protein Ag26584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB5B, member RAS oncogene family
Synonyms Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC032740
Gene Symbol RAB5B
Gene ID (NCBI) 5869
RRIDAB_2880862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61020
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

实验方案

Product Specific Protocols
WB protocol for RAB5B antibody 27403-1-APDownload protocol
IHC protocol for RAB5B antibody 27403-1-APDownload protocol
IF protocol for RAB5B antibody 27403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

bioRxiv

The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host

Authors - Seongok Kim
  • KD Validated
View Article
mouseWB

Proc Natl Acad Sci U S A

The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host

Authors - Seongok Kim
View Article
human,mouseIHC,WB,FC

Mol Hum Reprod

Hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis

Authors - Hairui Fan
View Article
mouseWB

Development

A crucial role for Arf6 in the response of commissural axons to Slit.

Authors - Mariko Kinoshita-Kawada
View Article