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验证数据展示

  • IF Staining of NIH/3T3 using CL488-27403

    IF Staining of NIH/3T3 using CL488-27403

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 RAB5B antibody (CL488-27403) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-27403

    FC experiment of NIH/3T3 using CL488-27403

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rab5b Polyclonal Antibody (CL488-27403)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB5B Polyclonal antibody

RAB5B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-27403

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 27403-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27403 targets RAB5B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen RAB5B fusion protein Ag26584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB5B, member RAS oncogene family
Synonyms Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC032740
Gene Symbol RAB5B
Gene ID (NCBI) 5869
RRIDAB_3672811
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61020
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB5B antibody CL488-27403Download protocol
FC protocol for CL Plus 488 RAB5B antibody CL488-27403Download protocol
Standard Protocols
Click here to view our Standard Protocols